GE RPN2510E Bedienungsanleitung PDF herunterladen (Seite 4)

2. Introduction

One of the most widely used techniques in the molecular biology field is the

immobilization of DNA and RNA onto a solid support membrane and subsequent

hybridizations with a specific single stranded probe, labeled to facilitate its

detection.

Using the GE Healthcare Hybridization Oven/Shaker ensures that the temperature

and shaking/rotation frequency, and hence the stringency of hybridization and

washing steps are rigidly controlled. This enables rapid and reproducible probing of

nucleic acids, and proteins immobilized on nylon and nitrocellulose membranes.

The GE Healthcare Hybridization Oven/Shaker is a multipurpose instrument

combining accurate temperature control with a choice of interchangeable

hybridization modes:

• Variablespeedrotisserie:holding7× 40 mm or 2 × 75 and 2 × 40 mm

hybridization bottles.

• Avariablespeedplatformshakerfor‘box’hybridizations,depurination,

denaturation and neutralization steps.

The instrument is:

• Economical: bottle hybridization minimizes probe volumes, reducing reagent

volumes and enhancing signal intensity.

• Precise: stringency of hybridization and washing steps are rigidly controlled,

ensuring reproducible results.

• Sensitive: validated protocols ensure optimal hybridization and washing steps,

enhancing multiple reprobing when using Hybond™ membranes.

• Safe: the double-glazed polycarbonate/acrylic door offers excellent thermal

insulation whilst minimizing radiation exposure.

• Convenient: small foot-print maximizes the use of limited laboratory space.

The instrument is suitable for use in conjunction with radiolabeled probes

using Rapid-hyb™ buffer, non-radioactive nucleic acid labeling and detection

systems such as AlkPhos Direct™, and protein labeling and detection systems

including ECL™, ECL Plus™ and ECL Advance™. Some protocols for use with these

applications are included in section 7.

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GE RPN2510E Bedienungsanleitung Seite 4